CURRICULUM VITAE



     
     
     

    JAMES EDWARD SMOLEN, PH.D.


    Department of Biochemistry and Cell Biology
    Rice University
    Houston, TX 77056

    (713) 348-4855

    E-Mail: jsmolen@rice.edu

    Education:

    Sept. 1967-June 1971 Harvard College, Cambridge, MA
    Graduated magna cum laude in Biochemistry Received A.B. degree June 1971

    Sept. 1971-Sept. 1976 Graduate School of Arts and Science, Harvard University, Department of Biological Chemistry, Harvard Medical School, Boston, MA. Thesis title: "Plasma Membrane Markers of Guinea Pig Granulocytes: Effects of Phagocytosis and Surface Modifiers". Ph.D. degree awarded November, 1976.

    Positions:

    Oct. 1976-April 1981 Associate Research Scientist,
    Department of Medicine, Division of Rheumatology, New York University School of Medicine.

    April 1981-August 1982 Research Assistant Professor
    Department of Medicine, Division of Rheumatology, New York University School of Medicine.

    Sept. 1982-Nov. 1986 Associate Research Scientist
    Department of Pediatrics, Division of Hematology/Oncology, University of Michigan Medical Center.

    March 1983-Nov. 1986 Assistant Research Scientist
    Department of Pathology, University of Michigan School of Medicine.

    Nov. 1986-March 1996 Associate Professor Department of Pediatrics
    Division of Hematology/Oncology, University of Michigan Medical Center.

    Nov. 1986- March 1996 Associate Professor
    Department of Pathology, University of Michigan School of Medicine.

    Sept. 1989- March 1996 Research Scientist
    Department of Pediatrics, Division of Hematology/Oncology, University of Michigan Medical Center.

    March 1996-June 2002 Associate Professor
    Department of Pediatrics
    Baylor College of Medicine 

    July 2003 - Present  Senior Department Administrator
    Department of Biochemistry and Cell Biology
    Rice University 

    Grants Awarded:

    1978-1980 Fellow - Arthritis Foundation

    1981-1986 Senior Fellowship - Arthritis Foundation

    1980 Biomedical Research Support Grant, NYU School of Medicine, "The Role of Protein Carboxymethylation in the Initiation of PMN Responses"

    1980-1992 NIH R01 "The Initiation of Granulocyte Responses"

    1983-1990 NIH, Program Project, "Neutrophil Activation in Adult Respiratory Distress Syndrome"

    1983-1984 Biomedical Research Support Grant, University of Michigan, "The Initiation of Granulocyte Responses"

    1983-1984 Michigan Cancer Research Institute, "Isolation of Neutrophil Protein Kinase C"

    1984-1986 American Heart Association of Michigan, "Protein Kinases of Rabbit Neutrophils"

    1984-1985 Children's Leukemia Foundation of Michigan, "Intracellular Calcium Metabolism in Human Blood Phagocytes"

    1994-1998 NIH R01 "The Initiation of Granulocyte Responses"

    1998-Present NASA, "Spaceflight Immunodeficiency", Co-PI

    Awards and Fellowships:

    1978-1980 Fellow of the Arthritis Foundation

    1981-1986 Senior Fellow of the Arthritis Foundation

    Societies:

    New York Academy of Sciences

    American Society for Cell Biology

    American Federation for Clinical Research

    American Association of Pathologists

    American Society of Hematologists

    American Association for the Advancement of Science

    University Committees:

    1987 University of Michigan, Department of Pediatrics Internal Review Committee

    1988-1996 University of Michigan, Medical School Admissions Committee

    1991-1993 University of Michigan, Department of Pediatrics, Departmental Appointments, Promotions, Titles and Affirmative Action Committee

    INVITED TALKS (Partial List):

    1980 University of West Virginia, Department of Biochemistry

    1981 Gordon Conference, "Oxygen Radicals in Biology and Medicine"

    1982 University of Michigan, Department of Pediatrics

    1984 Chairman at FASEB session

    1985 Chairman at FASEB session

    1986 University of Texas Health Science Center, Department of Biochemistry

    1990 University College, London, England

    1991 Midwest AFCR, Satellite Mini-Symposium on Signal Transduction

    1992 Co-Chairman at FASEB session

    1994 Central Laboratory of the Netherlands, Amsterdam

    1995 Baylor College of Medicine, Houston TX

    Grant Reviews and Study Sections:

    1981 NIH site visit, University of Oregon

    1986 NIH, Biochemistry 2 Study Section, ad hoc member

    1989 NIH, Special Study Section for Neutrophil Program Projects

    1989 NIH, Special Study Section for Individual Proposal

    1991 NIH, ad hoc Review Committee for Alzheimer's Disease

    1984-present Review of Individual Proposals for National Science Foundation

    1990-Present Review of Individual Proposals for Veterans Administration

    1992 NIH, Special Study Sections for Individual Proposals

    1992 NIH, Special Study Sections for Program Projects

    1992 NIH, ad hoc Review Committee for AIDS

    1991- 1994 Veterans Administration - Infectious Diseases Merit Review Board

    1995-Present American Heart Association - - Immunology and Microbiology Peer Review
     

    Reviewer for Refereed Journals:

    Biochemical Pharmacology Biochimica et Biophysica Acta

    Blood Cancer Research

    European Journal of Hematology Infection and Immunity

    Journal of Biological Chemistry Journal of Cellular Physiology

    Journal of Immunology Journal of Leukocyte Biology

    Life Sciences Molecular Pharmacology

    Nature Pharmacology and Toxicology

    The Journal of Clinical Investigation American Journal of Pathology

    Journal of Infectious Diseases

    Teaching Experience:

    1990-1991 Microbiology 620 Small Group Sessions

    1991 Pathology 850 Seminar

    1993 Pathology 850 - Fall Term Instructor

    1996- Integrated Problem Solving -- Facilitator

    Postdoctoral Fellows:

    University of Michigan School of Medicine

    1984-1986 Pamela Petrequin, Ph.D.

    1984-1987 Kenneth Balazovich, Ph.D.

    1985-1988 Richard Axtell, M.D.

    1985-1986 Claude Sportes, M.D.

    1986-1989 Rebecca Sandborg, Ph.D.

    1989-1992 Joseph W. Francis, Ph.D.

    Baylor College of Medicine

    1997-1998 Bernhard Allgier, M.D.

    2001-2002 Zhiji Li, M.D.

    Medical and Undergraduate Student Laboratory Training (Partial List):

    New York University School of Medicine

    1980 - 1982 Henry Edelson

    1982 Paul Noble

    University of Michigan

    1986 - 1987 Dana Bartone

    1988 - 1989 Eun Kyu Koh

    1989 Barbara Kuczynski

    1989 William Land

    1989 - 1990 Alan Woronoff

    1990 Rob Smith

    1990 - 1992 David Margolis

    1991 Michelle Mantel

    1991 Malay Mody

    1992 Kavitha Nagaprakash

    1993 Ronald Hessler

    1994 Scott French

    Baylor College of Medicine

    1998 Cody Koch

    BIBLIOGRAPHY

    Scientific Reports In Refereed Journals:

    1. Smolen, J.E., and Shohet, S.B.: Permeability Changes Induced by Peroxidation in Liposomes Prepared from Human Erythrocyte Lipids. J. Lipid Res. 15:273,280, 1974.

    2. Smolen, J.E., and Shohet, S.B.: Remodeling of Granulocyte Membrane Fatty Acids During Phagocytosis. J. Clin. Invest. 53:726-734, 1974.

    3. Smolen, J.E., and Weissmann, G.: Mg-ATPase as a Membrane Ecto-Enzyme of Human Polymorphonuclear Leukocytes: Inhibitors, Activators and Response to Phagocytosis. Biochim. Biophys. Acta 512:525-538, 1978.

    4. Smolen, J.E., and Weissmann, G.: Effects of Indomethacin, 5,8,11,14-Eicosatetraynoic Acid, and p-Bromophenacyl Bromide on Lysosomal Enzyme Release and Superoxide Anion Generation by Human Polymorphonuclear Leukocytes. Biochem. Pharmacol. 29:533-538, 1979.

    5. Smolen, J.E., and Karnovsky, M.L.: The Effect of Phagocytosis on Guinea Pig Granulocyte Membrane Markers. J. Cell Physiol. 102:71-80, 1980.

    6. Smolen, J.E., and Karnovsky, M.L.: The Effect of Surface Modifiers on an Ectoenzyme: Granulocyte 5'-Nucleotidase. Infec. Immunity. 28:475-485, 1980.

    7. Smolen, J.E., Korchak, H.M., and Weissmann, G.: Initial Kinetics of Lysosomal Enzyme Secretion and Superoxide Anion Generation by Human Polymorphonuclear Leukocytes. Inflammation 4:145-163, 1980.

    8. Smolen, J.E., Korchak, H.M., and Weissmann, G.: Increased Levels of Cyclic Adenosine 3', 5'-Monophosphate in Human Polymorphonuclear Leukocytes After Surface Stimulation. J. Clin. Invest. 65:1077-1085, 1980.

    9. Smolen, J.E., and Weissmann, G.: Stimuli Which Provoke Secretion of Azurophil Enzymes from Human Neutrophils Induce Increments in Adenosine Cyclic 3',5'-Monophosphate. Biochim. Biophys. Acta 672:197-206, 1981.

    10. Smolen, J.E., Korchak, H.M., and Weissmann, G.: The Roles of Intracellular and Extracellular Calcium in Lysosomal Enzyme Release and Superoxide Anion Generation by Human Polymorphonuclear Leukocytes. Biochim. Biophys. Acta 677:512-520, 1981.

    11. Edelson, H.S., Kaplan, H.B., Korchak, H.M., Smolen, J.E., and Weissmann, G.: Dissociation by Piroxicam of Degranulation and Superoxide Anion Generation from Decrements in Chlortetracycline Fluorescence of Activated Human Neutrophils. Biochem. Biophys. Res. Comm. 104:247-253, 1982.

    12. Korchak, H.M., Eisenstat, B.A., Smolen, J.E., Dunham, P.B., and Weissmann, G.: Stimulus-Response Coupling in the Human Neutrophil: The Role of Anion Fluxes in Degranulation. J. Biol. Chem. 257:6916-6922, 1982.

    13. Smolen, J.E., and Weissmann, G.: The Effect of Various Stimuli and Calcium Antagonists on the Fluorescence Response of Chlorotetracycline-Loaded Human Neutrophils. Biochim. Biophys. Acta 720:172-180, 1982.

    14. Serhan, C.N., Broekman, H.J., Korchak, H.M., Smolen, J.E., Marcus, A.J., and Weissmann, G.: Changes in Phosphatidylinositol and Phosphatidic Acid Accumulation in Stimulated Human Neutrophils: Relationship to Calcium Mobilization, Aggregation and Superoxide Radical Generation. Biochim. Biophys. Acta 762:420-428, 1983.

    15. Smolen, J.E., Eisenstat, B.A., and Weissmann, G.: The Fluorescence Response of Chlorotetracycline-Loaded Human Neutrophils. Correlations with Lysosomal Enzyme Release and Evidence for a "Trigger Pool" of Calcium. Biochim. Biophys. Acta 717:422-431, 1982.

    16. Serhan, C.N., Radin, A., Smolen, J.E., Korchak, H., Samuelsson, B., and Weissmann, G.: Leukotriene B4 is a Complete Secretagogue in Human Neutrophils: A Kinetic Analysis. Biochem. Biophys. Res. Comm. 107:1006-1012, 1982.

    17. Smolen, J.E., Korchak, H.M., and Weissmann, G.: Kinetics of Degranulation of Human Neutrophils as Measured by Quenching of 9-aminoacridine. Biochim. Biophys. Acta 762:145-153, 1983.

    18. Smolen, J.E., Noble, P., Freed, R., and Weissmann, G.: Metabolic Requirements for Maintenance of the Chlortetracycline-Labelled Pool of Membrane-Bound Calcium in Human Neutrophils. J. Cell. Physiol. 117:415-422, 1983.

    19. Smolen, J.E.: The Fluorescence Response of Chlortetracycline-Loaded Human Neutrophils is Modulated by Prostaglandin E1, But Not by Cyclic Nucleotides. FEBS Lett. 163:119-123, 1983.

    20. Smolen, J.E., and Geosits, S.J.: Human Neutrophil Phosphodiesterase: Calmodulin-Insensitivity and Other Properties. Inflammation 8:193-199, 1984.

    21. Smolen, J.E.: The "Lag Period" for Superoxide Anion Generation and Lysosomal Enzyme Release from Human Neutrophils. The Effects of Calcium Antagonists and Anion Channel Blockers. J. Lab. Clin. Med. 104:1-10, 1984.

    22. Long, M.W., Smolen, J.E., Boxer, L.A., and Szczepanski, P.: The Role of Phorbol Diesters in In Vitro Murine Megakaryocyte Colony Formation. J. Clin. Invest. 74:1686-1692, 1984.

    23. Borregaard, N., Boxer, L.A., Smolen, J.E., and Tauber, A.I.: Anomalous Neutrophil Granule Distribution in a Patient with Lactoferrin Deficiency: Pertinence to the Respiratory Burst. Am. J. Hematol. 18:255-260, 1985.

    24. Smolen, J.E., and Stoehr, S.J.: Micromolar Concentrations of Free Calcium Provoke Secretion of Lysozyme from Human Neutrophils Permeabilized with Saponin. J. Immunol. 134:1859-1865, 1985.

    25. Stoehr, S.J., Smolen, J.E., Holz, R.W., and Agranoff, B.W.: Inositol Trisphosphate Mobilizes Intracellular Calcium in Permeabilized Adrenal Chromaffin Cells. J. Neurochem. 46:637-640, 1985.

    26. Smolen, J.E., Stoehr, S.J., and Boxer, L.A.: Human Neutrophils Permeabilized with Digitonin Respond with Lysosomal Enzyme Release When Exposed to Micromolar Levels of Free Calcium. Biochim. Biophys. Acta 866:1-17, 1986.

    27. Petrequin, P.R., Todd III, R.F., Smolen, J.E., and Boxer, L.A.: Expression of Specific Granule Markers on the Cell Surface of Neutrophil Cytoplasts. Blood 67:1119-1125, 1986.

    28. Smolen, J.E., Todd III, R.F., and Boxer, L.A.: Expression of a Granule Membrane Marker on the Surface of Neutrophils Permeabilized with Digitonin. Correlations with Ca2+ Induced Degranulation. Am. J. Pathol. 124:281-285, 1986.

    29. Balazovich, K.J., Smolen, J.E., and Boxer, L.A.: Endogenous Inhibitor of Protein Kinase C: Association with Human Peripheral Blood Neutrophils but not with Specific Granule-Deficient Neutrophils or Cytoplasts. J. Immunol. 137:1665-1673, 1986.

    30. Balazovich, K.J., Smolen, J.E., and Boxer, L.A.: Ca2+ and Phospholipid-Dependent Protein Kinase (Protein Kinase C) Activity is not Necessarily Required for Secretion by Human Neutrophils. Blood 68:810-817, 1986.

    31. Smolen, J.E., and Stoehr, S.J.: Guanine Nucleotides Reduce the Free Calcium Requirement for Secretion of Granule Constituents from Permeabilized Human Neutrophils. Biochim. Biophys. Acta 889:171-178, 1986.

    32. Smolen, J.E., Stoehr, S.J., Trayner, A.E., and Sklar, L.A.: The Kinetics of Secretion from Permeabilized Human Neutrophils: Release of Elastase and Correlations with Other Granule Constituents and Right Angle Light Scatter. J. Leuk. Biol. 41:8-13, 1987.

    33. Sklar, L.A., Bokoch, G.M., Button, D., and Smolen, J.E.: Regulation of Ligand-Receptor Dynamics by Guanine Nucleotides: Real-Time Analysis of Interconverting States for the Neutrophil Formyl Peptide Receptor. J. Biol. Chem. 262:135-139, 1987.

    34. Sklar, L.A., Bokoch, G.M., Swann, W.N., Comstock, C., and Smolen, J.E.: Ligand-Receptor Dynamics and Signal Amplification in the Neutrophil. Int. J. Tissue React. 9:277-284, 1987.

    35. Bokoch, G.M., Sklar, L.A., and Smolen, J.E.: Guanine Nucleotide Regulatory Proteins as Transducers of Receptor-Stimulated Neutrophil Activation. Int. J. Tissue React. 9:285-293, 1987.

    36. Stoehr, S.J., and Smolen, J.E.: Osmotic Forces Are Not Critical for Ca2+-Induced Secretion from Permeabilized Human Neutrophils. J. Cell. Physiol. 135:169-178, 1988.

    37. Sandborg, R.R., and Smolen, J.E.: The Effects of Heavy Metal Cations and Sulfhydryl Reagents on Degranulation from Digitonin-Permeabilized Neutrophils. Biochim. Biophys. Acta 1010:330-337, 1989.

    38. Smolen, J.E., Stoehr, S.J., and Bartone, D.: Protein Kinase C is not Involved in Secretion by Permeabilized Human Neutrophils. Cell. Signal. 1:471-481, 1989.

    39. Francis, J.W., Smolen, J.E., Balazovich, K.J., Sandborg, R.R., and Boxer, L.A.: Calcium-Dependent Fusion Of The Plasma Membrane Fraction From Human Neutrophils With Liposomes. Biochim. Biophys. Acta 1025:1-9, 1990.

    40. Smolen, J.E., and Sandborg, R.R.: Ca2+-induced Secretion by Electropermeabilized Human Neutrophils. The Roles of Ca2+, Nucleotides and Protein Kinase C. Biochim. Biophys. Acta 1052:133-142, 1990.

    41. Stoehr, S.J., and Smolen, J.E.: Human Neutrophils Contain a Protein Kinase C-like Enzyme Which Utilizes GTP as Phosphate Donor. Cofactor Requirements, Kinetics, and Endogenous Acceptor Proteins. Blood 75:479-487, 1990.

    42. Axtell, R.A., Sandborg, R.R., Smolen, J.E., Ward, P.A., and Boxer, L.A.: Exposure of Human Neutrophils to Exogenous Nucleotides Causes Elevation in Intracellular Calcium, Transmembrane Calcium Fluxes, and an Alteration of a Cytosolic Factor Resulting in Enhanced Superoxide Production in Response of FMLP and Arachidonic Acid. Blood 75:1324-1339, 1990.

    43. Stoehr, S.J., Smolen, J.E., and Suchard, S.J.: Lipocortins Are Major Substrates for Protein Kinase C in Extracts of Human Neutrophils. J. Immunol. 144:3936-3945, 1990.

    44. Smolen, J.E., Stoehr, S.J., and Kuczynski, B.: Cyclic AMP Inhibits Secretion From Electroporated Human Neutrophils. J. Leuk. Biol. 49:172-179, 1991.

    45. Smolen, J.E., Stoehr, S.J., Kuczynski, B., Koh, E.K., and Omann, G.M.: Dual Effects of GTP­g­S on Secretion by Electroporated Human Neutrophils. Biochem. J. 279:657-664, 1991.

    46. Smolen, J.E., Kuczynski, B., Koh, E.K., Balazovich, K.J., and Woronoff, A.: "Depletion" of ATP by 2-Deoxyglucose. Secretion by Electroporated Human Neutrophils is not Restored by Re-addition of ATP. Biol. Signals 1:22-33, 1992.

    47. Francis, J.W., Balazovich, K.J., Smolen, J.E., and Boxer, L.A.: Human Neutrophil Annexin I Promotes Granule Aggregation and Modulates Ca2+-Dependent Membrane Fusion. J. Clin. Invest. 90:537-544, 1992.

    48. Brock, T.G., Nagaprakash, K., Margolis, D.I., Smolen, J.E.: Modelling Neutrophil Degranulation with Liposomes: Effect of Increasing the Complexity of Lipid Composition on Membrane Fusion. J. Memb. Biol. 141:139-148, 1994.

    49. Blackwood, R.A., Transue, A., Harsh, D.M., Brower, R.C., Zacharek, S.J., Smolen, J.E., and Hessler, R.J.: PLA2 promotes fusion between PMN specific granules and complex liposomes. J. Leuk. Biol. 59(5):663-670, 1996.

    50. Blackwood, R.A., Smolen, J.E., Hessler, R.J., Harsh, D.M., and Transue, A.: Development of aqueous-space mixing assay for fusion of granules and plasma membranes from human neutrophils. Biochem.J. 314(2):469-475, 1996.

    51. Blackwood, R.A., Smolen, J.E., Transue, A, Hessler, R.J., Harsh, D.M., Brower, R.C., and French, S.: Phospholipase D activity facilitates Ca2+-induced aggregation and fusion of complex liposomes . Am.J.Physiol.Cell Physiol. 272(4): C1279-C1285, 1997.

    52. Hessler, R.J., Blackwood, R.A., Brock, T.G., Francis, J.W., Harsh, D.M., and Smolen, J.E. Identification of glyceraldehyde-3-phosphate dehydrogenase as a Ca2+-dependent fusogen in human neutrophil cytosol. J. Leuk. Biol. 6: 331-336, 1998.

    53.  Smolen, J.E., Petersen, T.K., Koch, C., O'Keefe, S.J., Hanlon, W.A., Seo, S., Pearson, D., Fossett, M.C., and Simon, S.I. L-Selectin signaling of neutrophil adhsion and degranulation involves p38 MAP kinase. J. Biol. Chem., Vol. 275, Issue 21, 15876-15884, May 26, 2000.

    54. Smolen, J.E., Fossett, M.C., Joe, Y., Prince, J.E., Priest, E., Kanwar, S., and Smith, C.W. Antiorthostatic suspension for 14 days does not diminish the oxidative response of murine neutrophils. Aviation, Space and Environmental Medicine  71:1239-1247, 2000.

    55.  Lindsey, M., Wedin, K., Brown, M.D., Keller, C., Evans, A.J., Smolen, J.E., Burns, A.R., Rossen, R.D., Michael, L., and Entman, M. Matrix-Dependent Mechanism of Neutrophil-Mediated Release and Activation of Matrix Metalloproteinase 9 in Myocardial Ischemia/Reperfusion. Circulation 103(17):2181-2187, 2001.

    56.  Smolen, J.E., Hessler, R.J., Nauseef, W.M., Cogley, M., and Joe, Y. Identification and cloning of the SNARE proteins VAMP-2 and syntaxin-4 from HL-60 cells and human neutrophils. Inflammation 25(4):255-265, 2001.
     

    Books:

    None
     

    Chapters In Books And Reviews:

    1. Weissmann, G., Smolen, J.E., and Hoffstein, S.: Polymorphonuclear Leukocytes as Secretory Organs of Inflammation. J. Invest. Derm. 71:95-99, 1978.

    2. Weissmann, G., Korchak, H.M., Finkelstein, M., Smolen, J.E., and Hoffstein, S.: Uptake of Enzyme-Laden Liposomes by Animal Cells In Vitro and In Vivo. Ann. N.Y. Acad. Sci., 308:235-249, 1978.

    3. Smolen, J.E., and Weissmann, G.: The Granulocyte: Metabolic Properties and Mechanisms of Lysosomal Enzyme Release. In: Neutral Proteases of Human Polymorphonuclear Leukocytes (K. Havemann, and A. Janoff, eds.) Urban & Schwartzenberg, Baltimore and Munich, pp. 56-76, 1978.

    4. Weissmann, G., Korchak, H.M., Perez, H.D., Smolen, J.E., Goldstein, I.M., and Hoffstein, S.T.: The Secretory Code of the Neutrophil. J. Reticuloendothelial Soc., 26:687-700, 1979.

    5. Smolen, J.E. and Weissmann, G.: Polymorphonuclear Leukocytes. In: Arthritis and Allied Conditions ( McCarty, D.J., ed), Lea & Febiger, Philadelphia, pp. 282-295, 1979.

    6. Weissmann, G., Korchak, H.M., Perez, H.D., Smolen, J.E., Goldstein, I.M., and Hoffstein, S.T.: Leukocytes as Secretory Organs of Inflammation. In: Advances in Inflammation Research (Weissmann, G., Paoletti, R., and Samuelsson, B., eds.) Raven Press, New York, Vol. 1, P. 111, 1979.

    7. Smolen, J.E., and Weissmann, G.: Lysosomal Enzyme Release from Human Granulocytes is Inhibited by Indomethacin, ETYA, and BPB. In: Advances in Prostaglandin and Thromboxane Research (Ramwell, P., Paoletti, R., and Samuelsson, B., eds.) Raven Press, New York, Vol. 8, pp. 1695-1700, 1980.

    8. Weissmann, G., Smolen, J.E., and Korchak, H.M.: Release of Inflammatory Mediators from Stimulated Neutrophils. New Engl. J. Med. (Medical Seminars of the Beth Israel Hospital), 303:27-34, 1980.

    9. Korchak, H.M., Smolen, J.E., Eisenstat, B.A., Hoffstein, S.T., and Weissmann, G.: Activation of the Neutrophil: Role of Anion Fluxes in Neutrophil Stimulation. Ann. N.Y. Acad. Sci., 358:346-347, 1980.

    10. Smolen, J.E., Korchak, H.M., and Weissmann, G.: "The Secretory Code" of the Neutrophil. Part VI. Activation of the Secretory Process. In: Methods in Cell Biology (Hand, A.R., and Oliver, C., eds.), Academic Press, New York, Vol. 23 pp. 461-481, 1981.

    11. Weissmann, G., Smolen, J.E., and Korchak, H.M.: The Role of Calcium in Secretion of Inflammatory Mediators from Neutrophils. Seminars in Arthritis and Rheumatism, 11:84-86, 1981.

    12. Hoffstein, S.T., Korchak, H.M., and Smolen, J.E.: Mechanisms of Degranulation. In: Advances in Immunopharmacology (Hadden, J. et al, eds), Pergamon Press, Oxford and New York, pp. 197-205, 1981.

    13. Weissmann, G., Korchak, H.M., Perez, H.D., Smolen, J.E., Goldstein, I.M., and Hoffstein, S.T.: Neutrophils as Secretory Organs. In: Membranes, Molecules, Toxins and Cells (Bloch, K., Bolis, L., and Tosteson, D.C., eds.) PSG Publishing Co., Inc., Boston, Bristol, London, pp. 175-191, 1981.

    14. Smolen, J.E., and Weissmann, G.: The Secretion of Lysosomal Enzymes from Human Neutrophils: The First Events in Stimulus-Secretion Coupling. In: Lysosomes and Lysosomal Storage Diseases (Callahan, J.W., and Lowden, J.A., Eds.), Raven Press, New York, pp. 31-62, 1981.

    15. Smolen, J.E., Korchak, H.M., and Weissmann, G.: Stimulus-Secretion Coupling in Human Polymorphonuclear Leukocytes. In: The Secretory Process (M. Cantin, Ed.) Karger Medical and Scientific Publishers (In Press).

    16. Weissmann, G., Smolen, J.E., Korchak, H.M., and Hoffstein, S.T.: The Secretory Code of the Neutrophil. In: Cellular Interactions (Dingle and Gordon, eds.) Elsevier/North-Holland and Biomedical Press, pp. 15-31, 1981.

    17. Weissmann, G., Serhan, C., Smolen, J.E., Korchak, H.M., Friedman, R., and Kaplan, H.B.: Stimulus-Secretion Coupling in the Human Neutrophil: The Role of Phosphatidic Acid and Oxidized Fatty Acids in the Translocation of Calcium. In: Advances in Prostaglandin, Thromboxane and Leukotriene Research (Samuelsson, B., and Paoletti, R., eds.) Raven press, New York, Vol. 9, pp. 259-272, 1982.

    18. Serhan, C., Smolen, J.E., Korchak, H.M., and Weissmann, G.: Leukotriene B4 is a Complete Secretagogue: Calcium Translocation in Liposomes and Neutrophil Activation. In: Advances in Prostaglandin, Thromboxane and Leukotriene Research (Samuelsson, B., and Paoletti, R., eds.) Raven Press, New York. Vol. 10 pp. 259-273, 1982.

    19. Weissmann, G., Serhan, C., Korchak, H.M., Smolen, J.E., Broekman, M.J., and Marcus, A.J.: Neutrophils Generate Phosphatidic Acid, an "Endogenous Calcium Ionophore", Before Releasing Mediators of Inflammation. Trans. Assoc. Am. Physician Vol. XCIV:357-365, 1982.

    20. Smolen, J.E., and Boxer, L.A.: Disorders of Stimulus-Response Coupling in Neutrophils. In: Clinics in Laboratory Medicine (Dowgun, K., ed.) W.B. Saunders Company, Philadelphia, Vol. 3(4) pp. 779-800, 1983.

    21. Weissmann, G., Serhan, C., Korchak, H.M., and Smolen, J.E.: Mechanisms of Mediator Release from Neutrophils. Adv. Exp. Med. Biol. 172:527-552, 1984.

    22. Smolen, J.E.: Secretion from Neutrophils: A Critique of Modern In Vitro Techniques. In: The Secretory Process, Vol. 3. "In Vitro Methods for Studying Secretion. (Poisner, A.M., and Trifaro, J.M.) Elsevier, Amsterdam. pp. 29-43, 1987.

    23. Smolen, J.E.: Molecular Mechanisms of Leukocyte Stimulation: Contributions of Permeabilized Cell Systems. In: Leukocyte Emigration and Its Sequelae (Movat, H.Z., Ed.) Karger AG pp 140-149, 1987.

    24. Boxer, L.A., and Smolen, J.E.: Neutrophil Granule Constituents and Their Release in Health and Disease. In: Hematology /Oncology Clinics. Phagocytic Defects I, (Curnette, J.T., III, Ed.), W.B. Saunders Co., pp 101-134, 1988.

    25. Sandborg, R.R., and Smolen, J.E.: Early Events in Leukocyte Activation. In: Lab. Invest. 59:300-320, 1988.

    26. Smolen, J.E.: Characteristics and Mechanisms of Secretion by Neutrophils. In: The Neutrophil: Cellular Biochemistry and Physiology (Hallett, M.B., Ed.) CRC Press Inc., Boca Raton, pp 23-61, 1989.

    27. Sandborg, R.R., and Smolen, J.E.: Biology of Disease. Early Biochemical Events in Leukocyte Activation. In: Pathology Reviews (Rubin E., and Damjanov, I., Eds.), Humana Press, Clifton, NJ, pp. 133-153, 1989.

    28. Smolen, J.E., and Boxer, L.A.: Functions of Neutrophils. In: Hematology. 4th Edition (Williams, W.J., Beutler, E. Erslev, A.J., and Lichtman, M.A., Eds.), Chapter 83, McGraw-Hill, pp. 780-794, 1990.

    29. Johnson, K.J., Varani, J., and Smolen, J.E.: Neutrophil activation and function in health and disease. In: Granulocyte Responses to Cytokines. Basic and Clinical Research, edited by Coffey, R.G. New York: Marcel Dekker, Inc., pp. 1-46, 1992.

    30. Smolen, J.E.,: Neutrophil signal transaction: Calcium, kinases, and fusion. In: The Journal of Laboratory and Clinical Medicine, 120:527-532, 1992.

    31. Smolen, J.E. and Boxer, L.A.: Functions of neutrophils. In: Williams Hematology, edited by Beutler, E., Lichtman, M.A., Coller, B.S. and Kipps, T.J. New York, McGraw-Hill, pp. 779-797, 1995.

    32. Smolen, J.E. and Boxer, L.A.: Functions of neutrophils.  In: Williams Hematology, 6th Edition, edited by Beutler, E., Lichtman, M.A., Coller, B.S., Kipps, T.J., and Seligsohn, U., McGraw-Hill, New York; pp. 761-784, 2001.

    Abstracts, Preliminary Communications, Panel Discussions:(Partial List)

    1. Smolen, J.E., Stoehr, S.J., and Boxer, L.A.: Partial Resealing of Neutrophils Previously Permeabilized with Digitonin. J. Cell. Biol., 99:331a, 1984.

    2. Balazovich, K.J., Smolen, J.E., and Boxer, L.A.: Ca2+ and Phospholipid-Dependent "Protein Kinase C" Activity is Not Necessarily Required for Stimulus Secretion Coupling in Human Neutrophils. Blood, 64:64a, 1984.

    3. Smolen, J.E.: "Contributions of Permeabilized Neutrophils to an Understanding of Stimulus-Secretion Coupling". Invited talk at Federation of American Societies for Experimental Biology, April 21-26, 1985.

    4. Stoehr, S.J., Smolen, J.E., Holz, R.W., and Agranoff, B.D.: Inositol Trisphosphate Mobilizes Intracellular Ca2+ in Permeabilized Adrenal Chromaffin Cells. Clin. Res., 33:316a, 1985.

    5. Petrequin, P.R., Todd III, R.F., Smolen, J.E., and Boxer, L.A.: Expression of Specific Granule Markers on the Cell Surface of Neutrophil Cytoplasts. Clin. Res., 33:385a, 1985.

    6. Balazovich, K.J., Curnutte, J., Petrequin, P., Smolen, J.E., and Boxer, L.A.: An Inhibitor of Protein Kinase C May Be Associated With Specific Granules of Human Peripheral Blood Neutrophils. Clin. Res., 33:543a, 1985.

    7. Smolen, J.E., Todd III, R.F., Boxer, L.A., and Rich, A.M.: Calcium Induced Lysosomal Enzyme Release from Permeabilized Human Neutrophils Results in Cell Surface Expression of Specific Granule Components. Clin. Res., 33:513a, 1985.

    8. Smolen, J.E.: Guanine Nucleotides Reduce the Calcium Requirement for Granule Enzyme Secretion from Permeabilized Human Neutrophils. J. Cell Biol., 101:293a, 1985.

    9. Curnutte, J..T., Mayo, L.A., Stoehr, S.J., Balazovich, K.J., Boxer, L.A., and Smolen, J.E.: Enhanced Superoxide Production in a Patient Whose Neutrophils Have Elevated Levels of Intracellular Calcium and Protein Kinase C Activity. Blood, 66:195a, 1985.

    10. Smolen, J.E., Stoehr, S.J., and Sklar, L.A.: The Kinetics of Secretion from Permeabilized Human Neutrophils: Release of Elastase and Changes in Right Angle Light Scatter (invited talk at Federation of the American Societies for Experimental Biology; April 13-18, 1986). Fed. Proc., 45:3062a, 1986.

    11. Sklar, L.A., Bokoch, G.M., Button, D., and Smolen, J.E.: Regulation of Ligand-Receptor Dynamics by Guanyl Nucleotides: Rapidly Interconverting States for the Neutrophil Formyl Peptide Receptors. Fed. Proc., 45:5705a, 1986.

    12. Boxer, L.A., Balazovich, K.J., Curnutte, J.T., and Smolen, J.E.: Regulators and the Role of Protein Kinase C in Human Neutrophil Activation. Fed. Proc., 45:5708a, 1986.

    13. Traynor Kaplan, A.E., Harris, A.L., Omann, G.M., Smolen, J.E., and Sklar, L.A.: Transient Rise in Lysophosphoinositides concomitant with Neutrophil Activation. Fed. Proc. 46:1685a, 1987.

    14. Stoehr, S.J., and Smolen, J.E.: Effect of Solutes and Osmolarity on Neutrophil Secretion. Fed. Proc. 46:4179a, 1987.

    15. Axtell, R.A., Boxer, L.A., Smolen, J.E., Sandborg, R.R., and Ward, P.A.: Effect Of Nucleotides On Human Neutrophil Intracellular Calcium And Superoxide Production. Blood 70:186, 1987.

    16. Stoehr, S.J., Balazovich, K.J., and Smolen, J.E.: Protein Kinase C of Human Neutrophils Utilizes GTP as an Alternate Phosphate Donor. Clin. Res. 36:420a, 1988.

    17. Sandborg, R., and Smolen, J.E.: Heavy Metals Induce Granule Secretion from Permeabilized Neutrophils. FASEB J. 2:1584a, 1988.

    18. Axtell, R.A., Smolen, J.E., and Boxer, L.A.: Human Neutrophils Require Adenine Nucleotides for Full Activity of the Soluble Component of the NADPH Oxidase. Clin. Res. 36:839A, 1988.

    19. Axtell, R.A., Smolen, J.E., and Boxer, L.A.: Human Neutrophils (PMN) Require Adenine Nucleotides for Full Activity of the Soluble Component of the NADPH Oxidase. Blood 72:141a, 1988.

    20. Stoehr, S.J., Suchard, S.J., and Smolen, J.E.: Lipocortin I and II are the Major Substrates for Protein Kinase C in Extracts of Human Neutrophils. Blood 72:153a, 1988.

    21. Axtell, R.A., Smolen, J.E., and Boxer, L.A.: Purine Nucleotides Modulate Superoxide Production On Both Sides Of The Plasma Membrane. Pediatric Research, May, 1989.

    22. Stoehr, S.J., Smolen, J.E., and Suchard, S.J.: Lipocortins I and II are Major Substrates of Neutrophil Protein Kinase C In Vitro. FASEB J. 3:A962, 1989.

    23. Smolen, J.E., and Balazovich, K.J.: Characteristics of Ca2+-Induced Secretion by Electropermeabilized Human Neutrophils. FASEB J. 3:A1087, 1989

    24. Smolen, J.E., and Kuczynski, B.: Cyclic AMP Inhibits Secretion from Electroporated Human Neutrophils. FASEB J. 4:A693, 1990.

    25. Stoehr, S.J., Smolen, J.E., and Suchard, S.J.: A Lipocortin VI-like Protein is Phosphorylated in Electroporated Human Neutrophils. Clin. Res. 38:270A, 1990.

    26. Suchard, S.J., Burton, M.J., Stoehr, S.J., and Smolen, J.E.: Neutrophil Thrombospondin Receptor Expression Coincides with the Release of a Subpopulation of Specific Granules. J. Cell Biol. 111:50a, 1990.

    27. Smolen, J.E., Stoehr, S.J., Kuczyuski, B., and Koh, E.K.: Dual Effects of GTP-g-S on Secretion by Electroporated Human Neutrophils. J. Cell Biol. 111:76a, 1990.

    28. Francis, J.W., Stoehr, S.J., Margolis D.I., Boxer, L.A., and Smolen, J.E.: Glyceraldehyde 3 Phosphate Dehydrogenase Promotes Ca2+-Dependent Fusion of Liposomes and Secretion in Permeabilized Neutrophils. J. Cell Biol. 111:77a, 1990.

    29. Francis, J.W., Balazovich, K.J., Smolen, J.E., and Boxer, L.A.: Human Neutrophil Annexin I (Lipocortin I) is an Abundant Cytosol Protein that Promotes Ca2+-Dependent Fusion of Membranes. Clin. Res. 39:352a, 1991.

    30. Francis, J.W., Balazovich, K.J., Boxer, L.A., and Smolen, J.E.: Annexin I Promotes Contents-Mixing of Liposomes at Low Micromolar Calcium Concentrations in the Presence of Diacylglycerol. Clin. Res. 40:170a, 1992.

    31. Brock, T.G., Nagaprakash, K., and Smolen, J.E.: Modeling Neutrophil Degranulaiton: Effect Of Increasing Lipid Complexity On Ca2+-Mediated Fusion Of Liposomes. Clin. Res. 41:173a, 1993.

    32. Blackwood, R.A., Smolen, J.E., Transue, A., Harsh, D.M., and Hessler, R.J. Development of a simple universal contents-mixing assay to study fusion between phospholipid vesicles and PMN membranes. J.Invest.Med. 43:296a 1995.

    33. Blackwood, R.A., Smolen, J.E., Transue, A., Harsh, D.M., Hessler, R.J., and Brower, M.S. Phospholipase D activity facilitates Ca2+-induced fusion of phospholipid vesicles constructed to resemble PMN plasma membrane. J.Invest.Med. 43:296a 1995.

    34. Hessler, R.J., Blackwood, R.A., Brock, T.G., Francis, J.W., Harsh, D.M., and Smolen, J.E. Identification of glyceraldehyde-3-phosphate dehydrogenase as a Ca2+-dependent fusogen in human neutrophil cytosol. Molec. Biol. Cell 7:615a 1996.

    35. Smolen, J.E., Hessler, R.J., Nauseef, W.M., Cogley, M, and Joe, Y., Identification and cloning of the fusion protein VAMP-2 in HL-60 cells and human neutrophils. Molec. Biol. Cell 8:295a 1997.

    36. Smolen, J.E., Petersen, T.K., Koch, C., O'Keefe, S.J., Seo, S., Fossett, M.C., and Simon, S.I. L-Selectin signaling of neutrophil adhesion and degranulation involves p38 MAP Kinase. Mol.Biol.Cell 10:318a-318a, 1999.

    07/26/2003
    Visitors:

Click Here!